Transparent Exopolymer Particles Research Articles

Distribution of polysaccharidic and proteinaceous gel−like particles in three cyclonic eddies in the Eastern Tropical North Atlantic

Transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP), two prominent classes of gel−like particles in the ocean primarily produced by phytoplankton, play crucial roles in ecological and biogeochemical processes, influencing microbial nutrition, growth, and particle aggregation. The distribution of these particles is intricately linked to the spatiotemporal dynamics of phytoplankton. Mesoscale cyclonic eddies (CEs) are known to stimulate phytoplankton growth and influence particle transport, but their effects on TEP and CSP remain to be determined. In the Eastern Tropical North Atlantic (ETNA), we examined three CEs: one off the Mauritanian coast during summer (Mau), one offshore during winter (Sal), and another near Brava island during winter. Mau and Brava CEs were in their intensification/maturity phase, while the Sal CE was in its decay phase. Both TEP and CSP concentrations correlated with primary productivity, but TEP increased with chlorophyll−a concentration, whereas elevated CSP coincided also with the highest abundance of pico−nanophytoplankton (<20 µm), mainly Synechococcus. Both gels exhibited a positive correlation with bacterial biomass production, indicating their consumption by heterotrophic bacteria. TEP total area in the epipelagic waters of all CEs (Mau, Brava, and Sal) was elevated compared to surrounding waters, with on average 4, 2.5, and 1.6−fold higher values, respectively. However, no significant difference in TEP size distribution was observed within any CEs and their surroundings. Similarly, CSP total area increased in the epipelagic waters of Mau and Brava CEs, with on average 5 and 2.4−fold higher values, respectively, compared to surrounding waters. CSP particles were notably larger in these two eddies, while the Sal CE showed no significant difference from surrounding waters in CSP abundance and size. Overall, TEP and CSP exhibited distinct responses to CEs, with increased concentrations during their intensification/maturation stage and remineralization dominating during their decaying stage.

Functions of extracellular polymeric substances in partitioning suspended and sinking particles in the upper oceans of two open ocean systems

AbstractMarine particle dynamics and carbon export, involving extracellular polymeric substances (EPS) like transparent exopolymer particles (TEP) and Coomassie Brilliant Blue‐stained particles (CSP), are poorly understood. Although TEP adhesive properties may enhance carbon export by facilitating aggregate formation, their low density can also enhance particle suspension. Factors influencing TEP regulation of particle dynamics remain unclear. To investigate EPS contributions to particle dynamics, we investigated ratios of TEP to particulate organic carbon (POC) and of CSP to POC in suspended and sinking particles collected with marine snow catchers. Samples were collected in a subarctic region near Hokkaido during a spring phytoplankton bloom and in the oligotrophic, subtropical Kuroshio region. At Hokkaido, the mean TEP : POC ratio of sinking particles (0.075 μg Xeq. : μg C) was > 30× lower than in suspended particles (2.3), consistent with a model prediction of selective retention of buoyant TEP‐rich particles in the upper water column. In the Kuroshio region, sinking particles also contained fewer TEP than suspended particles; however, the TEP : POC ratio of sinking particles (1.0) was > 10× higher than at Hokkaido, suggesting that TEP constitute a significant carbon component of sinking particles. These findings indicate that TEP facilitate aggregation of high‐density particles and particle sinking in the Kuroshio region. Distributions of CSP : POC ratios between suspended and sinking particles resembled TEP : POC ratios in both regions, implying a significant contribution of CSP to particle dynamics. We propose that EPS have divergent effects on suspension and sinking of marine particles, which vary with particle composition and biogeochemical conditions.

Effect of biopolymer concentration on the kinetics of marine snow formation

Marine snow floc refers to coagulation of microbes and marine debris in the upper ocean layers, bound together by bio-polymers such as transparent exopolymer particles (TEP) secreted by microbes. The stickiness of TEP plays a crucial role in determining the rate of marine snow floc formation. Additionally, the effect of TEP on the size distribution of marine snow influences the sinking velocity of the flocs. Using a surrogate material system, we study the kinetics of marine snow using a custom-built experimental setup, which allows direct measurement of floc size, floc number density, and floc sinking velocity as a function of TEP concentration. By comparing the experimental floc size with Smoluchowski coagulation theory, we obtain stickiness index, which increases with TEP concentration first, reaches maximum around 0.3 g/L of TEP and decreases upon further increase in TEP concentration. The experimental sinking velocity scales with floc size as ws=adb, with b ranging from 0.57 to 0.68. The exponent is slightly higher than that of 0.5 expected in the Stokes limit. This study establishes a clear link between stickiness index, sinking velocity, and polymer concentration, providing valuable insights for modeling of marine snow dynamics in deep ocean conditions. These findings contribute to a better understanding of the kinetics of marine snow formation, essential for predicting carbon sequestration within the biological carbon pump.

Life in the PFAS lane: The impact of perfluoroalkyl substances on photosynthesis, cellular exudates, nutrient cycling, and composition of a marine microbial community

Perfluoroalkyl substances (PFAS) are of great ecological concern, however, exploration of their impact on bacteria-phytoplankton consortia is limited. This study employed a bioassay approach to investigate the effect of unary exposures of increasing concentrations of PFAS (perfluorooctane sulfonate (PFOS) and 6:2 fluorotelomer sulfonate (6:2 FTS)) on microbial communities from the northwestern Gulf of Mexico. Each community was examined for changes in growth and photophysiology, exudate production and shifts in community structure (16S and 18S rRNA genes). 6:2 FTS did not alter the growth or health of phytoplankton communities, as there were no changes relative to the controls (no PFOS added). On the other hand, PFOS elicited significant phototoxicity (p < 0.05), altering PSII antennae size, lowering PSII connectivity, and decreasing photosynthetic efficiency over the incubation (four days). PFOS induced a cellular protective response, indicated by significant increases (p < 0.001) in the release of transparent exopolymer particles (TEP) compared to the control. Eukaryotic communities (18S rRNA gene) changed substantially (p < 0.05) and to a greater extent than prokaryotic communities (16S rRNA gene) in PFOS treatments. Community shifts were concentration-dependent for eukaryotes, with the low treatment (5 mg/L PFOS) dominated by Coscinodiscophyceae (40 %), and the high treatment (30 mg/L PFOS) marked by a Trebouxiophyceae (50 %) dominance. Prokaryotic community shifts were not concentration dependent, as both treatment levels became depleted in Cyanobacteriia and were dominated by members of the Bacteroidia, Gammaproteobacteria, and Alphaproteobacteria classes. Further, PFOS significantly decreased (p < 0.05) the Shannon diversity and Pielou's evenness across treatments for eukaryotes, and in the low treatment (5 mg/L PFOS) for prokaryotes. These findings show that photophysiology was not impacted by 6:2 FTS but PFOS elicited toxicity that impacted photosynthesis, exudate release, and community composition. This research is crucial in understanding how PFOS impacts microbial communities.

Study on the onset mechanism of bio-blister degradation of polyolefin by diatom attachment in seawater

It is essential to develop a mechanism for lowering the molecular weight of polyolefins to achieve biodegradation in seawater. In this study, a polypropylene/polylactic acid blend sample was first subjected to photodegradation pretreatment, and it was confirmed that in pure water, the acid generated promotes the polypropylene degradation (autoxidation), while in alkaline seawater, the promotion was inhibited by a neutralization reaction. In the autoxidation of polyolefins in alkaline seawater, aqueous Cl− was also the inhibitor. However, we found that autoxidation could be initiated even in seawater by lowering the pH and using dissociation of ClOH (called blister degradation). The blister degradation mechanism enabled autoxidation, even in seawater, by taking advantage of the ability of diatoms to secrete transparent exopolymer particles (TEP) to prevent direct contact between the surface layer of polyolefins and alkaline seawater. We named blister degradation in seawater with diatoms as bio-blister degradation and confirmed its manifestation using linear low-density polyethylene (LLDPE)/starch samples by SEM, IR, DSC and GPC analysis.

Polysaccharide preferred minority-dominant community assembly and exoenzyme enrichment in transparent exopolymer particles: Implication for global carbon cycle in water

The ubiquitous transparent exopolymer particles (TEPs) are an important organic carbon pool and an ideal microhabitat for bacteria in aquatic environments. They play a crucial role in the global carbon cycle. Organic matter transformation and carbon turnover in TEPs strongly depend on the assembly of their associated bacterial communities and enzyme activity. However, the mechanisms of bacterial community assembly and their potential effects on the organic carbon cycle in TEPs are still unclear. In this study, we comparatively explored the community assembly of TEP-associated bacteria and bacterioplankton from surface freshwater using metagenomics. It was found that the bacterial community assembly in TEPs followed a minority-dominant rule and was governed by homogeneous selection. Pseudomonadota and Actinomycetota, which are responsible for polysaccharide degradation, serve as taxon-specific biomarkers among the abundant and diverse bacteria in TEPs. The network of TEP-associated bacteria displayed stronger robustness than that of bacterioplankton. Bin 76 (majorly Acinetobacter) was the overwhelmingly dominant taxa in TEPs, whereas there was no clearly dominant taxa in TEP-free water. Exoenzyme analysis showed that 64 out of 71 identified polysaccharide hydrolases were markedly linked with the dominant bin 76 in TEPs, while no such linkage was observed for bacterioplankton. Generally, Acinetobacter, which is capable of utilizing polysaccharides, is preferred to be assembled in TEPs together with high polysaccharide hydrolase activity. This may significantly accelerate the turnover of organic carbon in the giant global TEP pool. These findings are important for a deep understanding of the carbon cycle in water.

Mitigation of transparent exopolymer particles-induced membrane biofouling by the coupling process of magnetic ion exchange resin/ultrafiltration

The significant effects of transparent exopolymer particles (TEP) on membrane fouling have attracted the attention of several researchers. Finding an effective method for controlling membrane biofouling caused by TEP is essential for the long-term stable operation of membrane systems. The results of this experimental study show that the coupling process of magnetic ion exchange resin (MIEX)/Ultrafiltration (UF) can significantly mitigate membrane biofouling induced by TEP. Compared with the UF process, the irreversible growth rate of TEP on the membrane in the MIEX/UF process decreased by 75.9%, and the synergistic irreversible accumulation rate of TEP and bacteria decreased by 81.2%. The irreversible adhesion of bacteria to the membrane was significantly reduced, effectively inhibiting the formation of the primary level of biofilm and the development of biofouling. Further analysis showed that the MIEX inhibited the conversion of TEP precursors to TEP, and the quaternary ammonium salt functional groups on the surface of the MIEX sterilized bacteria and inhibited their proliferation in water. More importantly, because the interfacial forces between TEP/bacteria and MIEX were much higher than those between them and the UF membrane, the deposition of MIEX on the membrane surface not only prevented TEP and bacteria from reaching the membrane surface, but also removed the deposited TEP and bacteria from the membrane surface by hydraulic backwashing.

Enhanced microalgal growth and metabolites through co-cultivation with Escherichia coli in algal organic matter solution

Studies of how symbiotic bacteria interact with microalgae have been an area of interest for some time. However, there is still a knowledge gap on the role of algal organic matters in algal growth promotion and adaptive mechanisms of the co-cultures in medium containing high abundancy of dissolved algal organic matters. It remains unclear if high extra- and intra-cellular organic substances concentration influences algal development or subsequent algal organic matter productivity in the presence of bacteria. Therefore, this study aims to co-cultivate Escherichia coli NEB 5-alpha with broad range of marine and freshwater microalgae in fresh medium (T), soluble EPS (sEPS) and internal organic matter solution (IOM). Among screened species, only the growth of Cylindrotheca fusiformis and Chlorella vulgaris was promoted by E. coli at 1.15×106±2.08×104 cells mL−1 and 7.79×106±1.27×105 cells mL−1after 96 h. Xenic algal growth was further enhanced about 10 % more utilizing sEPS and IOM. However, the net EPS accumulation of the cells cultivated in sEPS and IOM was comparatively lower than that of the cells in T medium due to metabolite consumption. Elevation in the transparent exopolymer particles, total chlorophyll, total carbohydrate, total protein and lipid productivity up to 3.54-fold by cultivating the co-cultures in sEPS and IOM indicated an alteration of the microalgae's physiology. This is a new paradigm on how algal extracts promote xenic algal growth and metabolite production which pave a way for cost-effective biotechnological applications by reusing the algae medium with abundant organic matters.

The mechanism of silica and transparent exopolymer particles (TEP) on reverse osmosis membranes fouling

Dissolved silica and transparent exopolymer particles (TEP) are the primary foulants in reverse osmosis (RO) desalinated brackish water and wastewater. In this study, we investigated the fouling properties of varying silica concentrations with TEP on the membrane surface and discovered a synergistic fouling effect between the silanol group (Si–OH) and the TEP carboxyl group (-COOH). The membrane fouling experiments showed that silica fouling approached saturation at 6 mM, with little variation in membrane flux as the silica concentration increased. Furthermore, the –OH functional group of the monosilicate molecule can chemically react with the -COO- functional group on the membrane surface to create hydrogen bonds, allowing monosilicate deposition directly on the membrane. Silica-silica interactions reacted with aggregates at high silica concentrations and joined with TEP to create a more substantial, more complex cross-linked network, resulting in severe membrane fouling. At pH 9, silica fouling was most severe due to the dramatic increase in the solubility of monosilicic acid dissolution in solution and the decreased polymerization rate. This work reveals the essential process of membrane fouling induced by silica and TEP, significantly increasing knowledge on silica-TEP fouling.

Mitigation of reverse osmosis membrane fouling by coagulation pretreatment to remove silica and transparent exopolymer particles

The dissolution of silica and transparent exopolymer particles (TEP) can deposit on the membrane surface and cause serious membrane fouling in reverse osmosis (RO) technology. Coagulation, as a common pretreatment process for RO, can effectively intercept pollutants and alleviate membrane fouling. In this study, FeCl3 and AlCl3 coagulants and polyacrylamide (PAM) flocculants were used to explore the optimal coagulation conditions to reduce the concentration of silica and TEP in the RO process. The results showed that the two coagulants had the best removal effect on pollutants when the pH was 7 and the dosage was 50 mg/L. Considering the proportion of reversible fouling after coagulation, the removal rate of pollutants, and the residual amount of coagulation metal ions, the best PAM dosage was 5 mg/L for FeCl3 and 1 mg/L for AlCl3. After coagulation pretreatment, the Zeta potential decreased, and the particle size distribution increased, making pollutants tend to aggregate, thus effectively removing foulants. The removal mechanisms of pollutants by coagulation pretreatment were determined to be adsorption, electric neutralization and co-precipitation. This study determined the best removal conditions of silica and TEP by coagulation and explored the removal mechanism.

A Comprehensive Analysis of the Impact of Inorganic Matter on Membrane Organic Fouling: A Mini Review.

Membrane fouling is a non-negligible issue affecting the performance of membrane systems. Particularly, organic fouling is the most persistent and severe form of fouling. The complexation between inorganic and organic matter may exacerbate membrane organic fouling. This mini review systematically analyzes the role of inorganic matter in membrane organic fouling. Inorganic substances, such as metal ions and silica, can interact with organic foulants like humic acids, polysaccharides, and proteins through ionic bonding, hydrogen bonding, coordination, and van der Waals interactions. These interactions facilitate the formation of larger aggregates that exacerbate fouling, especially for reverse osmosis membranes. Molecular simulations using molecular dynamics (MD) and density functional theory (DFT) provide valuable mechanistic insights complementing fouling experiments. Polysaccharide fouling is mainly governed by transparent exopolymer particle (TEP) formations induced by inorganic ion bridging. Inorganic coagulants like aluminum and iron salts mitigate fouling for ultrafiltration but not reverse osmosis membranes. This review summarizes the effects of critical inorganic constituents on fouling by major organic foulants, providing an important reference for membrane fouling modeling and fouling control strategies.

Unexpected significance of magnesium ion in the coupling effect of mixed divalent cations on polysaccharide membrane fouling

Divalent cations and organics are two main types of foulants in feed water to the membrane processes and cations often bind to organic foulants which significantly affects fouling. Extensive efforts have been devoted to exploring the influence of single cation on organic fouling while there are diverse cations in the feed. Thus, it is essential to understand the coupling influence of different divalent cations on fouling development. In this study, sodium alginate was chosen as a model foulant to investigate the effect of different Ca2+/Mg2+ ratios on its fouling behavior. Filtration performances, the quantity and morphology of transparent exopolymer particles (TEP) derived from alginate were analyzed along with the morphology and structure of corresponding fouling layers. TEP measurements reveal that the Ca2+/Mg2+ ratio is crucial in the characteristics of TEP, which further determines the structure of the fouling layer. At a low Ca2+/Mg2+ ratio, the TEP are amorphous-TEP (a-TEP) with small and unconsolidated structure which forms a fouling layer under pressure with high hydraulic resistance. In contrast, interactions between polysaccharides at high Ca2+/Mg2+ ratio are much stronger and result in large and tight particulate-TEP (p-TEP). Such p-TEP lead to a fouling layer with high porosity and low hydraulic resistance. The role of Mg2+ in polysaccharide fouling is more important than expected because it promotes the formation of a-TEP, which show a high fouling potential at high levels because more dense gel layer forms. This study suggests that the morphology and structure of TEP play crucial roles in polysaccharide fouling propensities. A-TEP at high levels needs more attention to ensure the successful operation of membrane systems in future work.

The importance of transparent exopolymer particles over ballast in determining both sinking and suspension of small particles during late summer in the Northeast Pacific Ocean

Gravitational sinking of particles is a key pathway for the transport of particulate organic carbon (POC) to the deep ocean. Particle size and composition influence particle sinking velocity and thus play a critical role in controlling particle flux. Canonically, sinking particles that reach the mesopelagic are expected to be either large or ballasted by minerals. However, the presence of transparent exopolymer particles (TEP), which are positively buoyant, may also influence particle sinking velocity. We investigated the relationship between particle composition and sinking velocity during the Export Processes in the Ocean from RemoTe Sensing (EXPORTS) campaign in the Northeast Pacific Ocean using Marine Snow Catchers. Suspended and sinking particles were sized using FlowCam for particle imaging, and their biogeochemical composition was assessed by measuring the concentration of particulate organic carbon (POC) and nitrogen, particulate inorganic carbon, biogenic and lithogenic silica, and TEP. Sinking fluxes were also calculated. Overall, both suspended and sinking particles were small (&amp;lt;51 μm, diameter) in this late summer, oligotrophic system. Contrary to expectation, the ratio of ballast minerals to POC was higher for suspended particles than sinking particles. Further, suspended particles showed TEP-to-POC ratios three times higher than sinking particles. These ratios suggest that TEP content and not ballast dictated whether particles in this system would sink (low TEP) or remain suspended (high TEP). Fluxes of POC averaged 4.3 ± 2.5 mmol C m−2 d−1 at 50 m (n = 9) and decreased to 3.1 ± 1.1 mmol C m−2 d−1 at 300–500 m (n = 6). These flux estimates were slightly higher than fluxes measured during EXPORTS with drifting sediment traps and Thorium-234. A comparison between these approaches illustrates that small sinking particles were an important component of the POC flux in the mesopelagic of this late summer oligotrophic system.

Irreversible membrane fouling caused by free TEP: Mitigation performance and mechanism of the integrated MIEX/UF process

Free transparent exopolymer particles (TEP) are an important type of TEP. Researchers have recognized the significant contribution of free TEP to irreversible membrane fouling when treating algae-laden water. Constructing a reasonable process to effectively decelerate membrane fouling caused by free TEP is necessary for the ultrafiltration of algae-laden water. Our experiments indicate that the integrated process of magnetic anion exchange resin (MIEX)/ultrafiltration (UF) can effectively impede the irreversible membrane fouling caused by free TEP. Compared with independent UF, after multi-cycle filtration, the growth rate of hydraulically and chemically irreversible fouling decreased by 40 % and 21 %, respectively, during the MIEX/UF process. The interfacial forces between free TEP and polyvinylidene fluoride (PVDF)/MIEX were determined using atomic force microscopy, and the results showed that the interfacial force of MIEX–free TEP was 4.3-fold higher than that of PVDF–free TEP. Although the adsorption of free TEP by MIEX partly reduced membrane fouling during the MIEX/UF process, the MIEX dynamic layer contributed the most to the alleviation of irreversible membrane fouling during MIEX/UF. Our experimental results provide a more feasible process design for effectively controlling the TEP-induced irreversible membrane fouling.

Polymeric carbohydrates utilization separates microbiomes into niches: insights into the diversity of microbial carbohydrate-active enzymes in the inner shelf of the Pearl River Estuary, China.

Polymeric carbohydrates are abundant and their recycling by microbes is a key process of the ocean carbon cycle. A deeper analysis of carbohydrate-active enzymes (CAZymes) can offer a window into the mechanisms of microbial communities to degrade carbohydrates in the ocean. In this study, metagenomic genes encoding microbial CAZymes and sugar transporter systems were predicted to assess the microbial glycan niches and functional potentials of glycan utilization in the inner shelf of the Pearl River Estuary (PRE). The CAZymes gene compositions were significantly different between in free-living (0.2-3 μm, FL) and particle-associated (>3 μm, PA) bacteria of the water column and between water and surface sediments, reflecting glycan niche separation on size fraction and selective degradation in depth. Proteobacteria and Bacteroidota had the highest abundance and glycan niche width of CAZymes genes, respectively. At the genus level, Alteromonas (Gammaproteobacteria) exhibited the greatest abundance and glycan niche width of CAZymes genes and were marked by a high abundance of periplasmic transporter protein TonB and members of the major facilitator superfamily (MFS). The increasing contribution of genes encoding CAZymes and transporters for Alteromonas in bottom water contrasted to surface water and their metabolism are tightly related with particulate carbohydrates (pectin, alginate, starch, lignin-cellulose, chitin, and peptidoglycan) rather than on the utilization of ambient-water DOC. Candidatus Pelagibacter (Alphaproteobacteria) had a narrow glycan niche and was primarily preferred for nitrogen-containing carbohydrates, while their abundant sugar ABC (ATP binding cassette) transporter supported the scavenging mode for carbohydrate assimilation. Planctomycetota, Verrucomicrobiota, and Bacteroidota had similar potential glycan niches in the consumption of the main component of transparent exopolymer particles (sulfated fucose and rhamnose containing polysaccharide and sulfated-N-glycan), developing considerable niche overlap among these taxa. The most abundant CAZymes and transporter genes as well as the widest glycan niche in the abundant bacterial taxa implied their potential key roles on the organic carbon utilization, and the high degree of glycan niches separation and polysaccharide composition importantly influenced bacterial communities in the coastal waters of PRE. These findings expand the current understanding of the organic carbon biotransformation, underlying the size-fractionated glycan niche separation near the estuarine system.

Effectiveness of ceramic ultrafiltration as pretreatment for seawater reverse osmosis

The Gulf Cooperation Council (GCC) region's considerable reliance on seawater reverse osmosis (SWRO) for freshwater production is amplifying with increasing population and industrial growth. Nonetheless, harsh seawater conditions in the region coupled with ineffective pretreatment have triggered frequent SWRO plant shutdowns, premature RO membrane and cartridge filter replacements, and low plant availability. To improve plant performance and sustainability, an effective pretreatment is needed; a promising option is the use of ceramic membranes. To investigate their effectiveness for handling variable seawater conditions experienced in the region — specifically harmful algal blooms (HAB) — this work presents a pilot scale ceramic UF (CUF) pretreatment study. Operational reliability and treated water quality of CUF was evaluated against existing polymeric MF and UF pretreatment in adjacent SWRO plants. CUF operated at full capacity during HAB season, compared to 30–40 % reduction for the MF/UF plants. Furthermore, it produced superior permeate quality, despite variations in feedwater conditions — for instance, it removed over 5× more Dissolved Organic Carbon (DOC) and 1.5× more Transparent Exopolymer Particles (TEP) than the polymeric membranes. These results indicate the potential for CUF as an effective pretreatment for SWRO to minimize plant downtime and ensure consistent permeate quality during all seawater conditions.

Spatio-temporal variations in organic carbon composition driven by two different major phytoplankton communities in the Ross Sea, Antarctica

The compositions of organic carbon could be important in determining biological carbon pump efficiency. However, little information on them in relation to each algal assemblage is currently available in the Ross Sea. Here, we investigated the seasonal variations in organic carbon composition and the relative abundance of each organic carbon, including particulate organic carbon (POC), dissolved organic carbon (DOC), and transparent exopolymer particles (TEPs), characterized by different algal groups in the Ross Sea. The average POC and DOC contributions to the total organic carbon (TOC = POC + DOC) were 13.8 ± 3.7 % and 86.2 ± 3.7 % in mid-January 2019 and 20.9 ± 4.1 % and 79.1 ± 4.1 % in February–March 2018, respectively. The carbon content of TEP (TEP-C) contributed 19.6 ± 11.7 % and 4.6 ± 7.0 % of POC and TOC in mid-January and 36.2 ± 14.8 % and 9.0 ± 6.7 % in February–March, respectively. We found that the organic carbon compositions were affected by seasonal variations in the phytoplankton bloom phase, physical characteristics, and phytoplankton community structure. DOC concentrations and contributions to the TOC increased as phytoplankton cells became senescent in mid-January and decreased in February–March when phytoplankton were relatively active. From February–March, the deepened mixed layer depth encouraged TEP formation, subsequently increasing the TEP contributions. Regardless of the sampling season, all organic carbon concentrations per unit Chl-a were significantly higher in P. antarctica–abundant groups. The DOC contributions to the TOC were correspondingly higher at the P. antarctica–abundant stations in mid-January, which indicates that P. antarctica could be also important in the DOC contributions in the Ross Sea. The rapid alteration in environmental characteristics and phytoplankton community structures in the Ross Sea due to climate change could affect the organic carbon pool at the euphotic layer which consequently could determine the efficiency of the biological pump.

Spatial and temporal occurrence of preformed nitrate anomalies in the subtropical North Pacific and North Atlantic oceans

Preformed nitrate (PreNO3) was formulated to act as a conservative tracer of ocean circulation after accounting for the stoichiometry of marine biochemical reactions involving oxygen and nitrate. However, PreNO3 anomalies (e.g. negative values) have been identified within the shallow subsurface of the subtropical ocean which still have yet to be fully explained. The mechanisms proposed to drive the formation of PreNO3 anomalies include: vertical export and remineralization of N-poor dissolved organic matter (DOM) or transparent exopolymer particles (TEP), and the actions of vertically migrating phytoplankton. In this study we use observations from the subtropical Pacific and Atlantic Oceans taken by twenty Biogeochemical Argo profiling floats to assess the spatiotemporal variability of negative subsurface and positive euphotic zone PreNO3 anomalies and their formation rates. After explicitly accounting for the magnitude of DOM cycling, residual euphotic zone positive PreNO3 anomalies and subsurface negative PreNO3 anomalies are consistently observed between 40°S and 40°N. The seasonal timing of euphotic zone positive residual PreNO3 anomaly formation in relation to subsurface negative residual PreNO3 anomalies suggests that both vertically migrating phytoplankton and the remineralization of N-deficient TEP likely contribute to the formation of residual PreNO3 anomalies, but with regional variance suggesting a potentially larger role for migrating phytoplankton in the North Pacific and TEP processes in the North Atlantic. Since migrators include large diatoms that produce ballasted organic matter while TEP may only sink shallowly before remineralization, further investigation into the mechanisms generating PreNO3 anomalies and their quantitative significance is needed to assess the future trajectory of the biological carbon pump in an expanding subtropical ocean ecosystem.

Suspended particulate matter-bound per- and polyfluoroalkyl substances (PFASs) in a river-coastal system: Possible correlation with transparent exopolymer particles.

The transport and ultimate fate of per- and polyfluoroalkyl substances (PFASs) are generally considered to be influenced by partitioning behavior between water, suspended particulate matters (SPM), and sediments. This study examined the distribution and partitioning of the PFASs in the water, SPM, and sediments in a densely populated urban river-coastal system. The total concentrations of eight PFASs (∑8 PFASs) in the water phase, SPM, and sediments varied from 0.59 to 7.40 ng/L, 0.54 to 9.08 ng/g, and 0.05 to 0.13 ng/g, respectively. The PFAS concentrations in the water and SPM phase decreased as the salinity increased, confirming contaminant inputs from the upstream of the river to the estuary zone. Notably, the positive correlation between SPM-bound PFASs and transparent exopolymer particles (TEPs) content, providing first evidence that TEPs may accumulate and concentrate more PFASs on the SPM. Collectively, this results offers useful information about roles of TEPs in determining environmental fate of PFASs.

Transparent exopolymer particle production by diatoms and its relation with CO2 flux in mangrove waters of Sundarban, West Bengal

Transparent exopolymer particle production by diatoms and its relation with CO2 flux in mangrove waters of Sundarban, West Bengal